Dégradation de micro-polluants organiques par des procédés biologiques innovants (thèse: 2014 - 2017)
Activités
Traitement des eaux
Micro-polluants
Bio-réacteur à
membrane
Biodégradation
Publications scientifiques au M2P2
2020
Camille Grandclement, Anne Piram, Marie-Eleonore Petit, Isabelle Seyssiecq, Isabelle Laffont-Schwob, et al.. Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 49-Hydroxy-diclofenac. Journal of Chemistry , 2020, 2020, pp.9789420. ⟨10.1155/2020/9789420⟩. ⟨hal-02564026⟩ Plus de détails...
Since bacterial consortia involved in conventional wastewater treatment processes are not efficient in removing diclofenac (DCF), an emerging pollutant frequently detected in water bodies, the identification of microorganisms able to metabolise this pharmaceutical compound is relevant. us, DCF removal was investigated using bacteria isolated from aqueous stock solutions of this micro-pollutant and identified as Bacillus and Brevibacillus species using 16S rRNA gene sequencing. A 100% DCF removal was achieved after 17 hours of experiment at 20°C in a nutrient medium; the biodegradation kinetic followed a pseudo-first order (k biol = 11 L·g SS −1 ·d −1). Quantitative assessment of DCF removal showed that its main route was biotic degradation. e main degradation product of DCF, 4′-hydroxy-diclofenac (4′-OH-DCF), was identified using liquid chromatography-electrospray ionisation high-resolution mass spectrometry. Since the ecotoxicological impact of 4′-hydroxy-diclofenac was not reported in the literature, the ecotoxicity of DCF and its metabolite were tentatively evaluated using Vibrio fischeri bioassays. Results from these tests showed that this metabolite is not more toxic than its parent compound and may hopefully be an intermediate product in the DCF transformation. Indeed, no significant difference in ecotoxicity was observed after 30 min between DCF (50 should be writtten in subscript all along the manuscript in EC 50 = 23 ± 4 mg·L −1) and 4′-hydroxy-diclofenac (EC 50 = 19 ± 2 mg·L −1). Besides, the study highlighted a limit of the Microtox ® bioassay, which is largely used to assess ecotoxicity. e bioluminescence of Vibrio fischeri was impacted due to the production of microbial activity and the occurrence of some carbon source in the studied medium.
Camille Grandclement, Anne Piram, Marie-Eleonore Petit, Isabelle Seyssiecq, Isabelle Laffont-Schwob, et al.. Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 49-Hydroxy-diclofenac. Journal of Chemistry , 2020, 2020, pp.9789420. ⟨10.1155/2020/9789420⟩. ⟨hal-02564026⟩
Camille Grandclement, Isabelle Seyssiecq, Anne Piram, Pascal Wong-Wah-Chung, Guillaume Vanot, et al.. From the conventional biological wastewater treatment to hybrid processes, the evaluation of organic micropollutant removal: A review. Water Research, 2017, 111, pp.297-317. ⟨10.1016/j.watres.2017.01.005⟩. ⟨hal-01456484⟩ Plus de détails...
Because of the recalcitrance of some micropollutants to conventional wastewater treatment systems, the occurrence of organic micropollutants in water has become a worldwide issue, and an increasing environmental concern. Their biodegradation during wastewater treatments could be an interesting and low cost alternative to conventional physical and chemical processes. This paper provides a review of the organic micropollutants removal efficiency from wastewaters. It analyses different biological processes, from conventional ones, to new hybrid ones. Micropollutant removals appear to be compound-and process-dependent, for all investigated processes. The influence of the main physico-chemical parameters is discussed, as well as the removal efficiency of different microorganisms such as bacteria or white rot fungi, and the role of their specific enzymes. Even though some hybrid processes show promising micropollutant removals, further studies are needed to optimize these water treatment processes, in particular in terms of technical and economical competitiveness.
Camille Grandclement, Isabelle Seyssiecq, Anne Piram, Pascal Wong-Wah-Chung, Guillaume Vanot, et al.. From the conventional biological wastewater treatment to hybrid processes, the evaluation of organic micropollutant removal: A review. Water Research, 2017, 111, pp.297-317. ⟨10.1016/j.watres.2017.01.005⟩. ⟨hal-01456484⟩
