This article deals with the extraction of neutral lipids andă antioxidants from enriched Spirulina platensis microalgae usingă supercritical carbon dioxide (CO2), and more particularly with theă influence of experimental conditions on extraction yields and kineticsă at laboratory and pilot scales. Preliminary studies were carried out ată laboratory scale before the establishment of an experimental design:ă extraction curves were plotted for different autoclave fill rates, andă under different conditions of pressure and temperature. Using a Responseă Surface Methodology, the significant influence of pressure on extractionă efficiency was highlighted. Surface responses showed that, in theă studied experimental field, mass loss increased when pressure,ă temperature, and CO2/microalgae mass ratio increased. Extract analysesă showed that oil extracts contained chlorophylls a and b, as well asă beta-carotene. Finally, larger-scale experiments were carried out withă batches of 1 and 50 kg (scale-factors of 100 and 5000, respectively) andă the results were consistent with those obtained at laboratory scale. (C)ă 2016 Elsevier B.V. All rights reserved.