Fractionnement supercritique appliqué à des composés d’intérêt industriel (thèse: 2013 - 2016)
Activités
CO2 supercritique,
Fractionnement supercritique
Publications scientifiques au M2P2
2017
M. Lobon-Iglesias, G. Giraud, D. Castel, C. Philippe, M. Debily, et al.. Diffuse intrinsic pontine gliomas (DIPG) at recurrence: is there a window to test new therapies in some patients?. Journal of Neuro-Oncology, 2017, 137 (1), pp.111-118. ⟨10.1007/s11060-017-2702-7⟩. ⟨hal-04515971⟩ Plus de détails...
Children with diffuse intrinsic pontine glioma (DIPG) need new and more efficient treatments. They can be developed at relapse or at diagnosis, but therefore they must be combined with radiotherapy. Survival of children after recurrence and its predictors were studied to inform the possibility to design early phase clinical trials for DIPG at this stage. Among 142 DIPG patients treated between 1998 and 2014, 114 had biopsy-proven DIPG with histone H3 status available for 83. We defined as long survivors' patients who survived more than 3 months after relapse which corresponds to the minimal life expectancy requested for phase I/II trials. Factors influencing post-relapse survival were accordingly compared between short and long-term survivors after relapse. Fifty-seven percent of patients were considered long survivors and 70% of them had a Lansky Play Scale (LPS) above 50% at relapse. Patients who became steroids-independent after initial treatment for at least 2 months had better survival after relapse (3.7 versus 2.6 months, p = 0.001). LPS above 50% at relapse was correlated with better survival after relapse (3.8 versus 1.8 months, p < 0.001). Patients with H3.1 mutation survived longer after relapse (4.9 versus 2.7 months, p = 0.007). Patients who received a second radiotherapy at the time of relapse had an improved survival (7.5 versus 4 months, p = 0.001). In the two-way ANOVA analysis, steroid-independence and LPS predicted survival best and the type of histone H3 (H3.1 or H3.3) mutated did not improve prediction. Survival of many DIPG patients after relapse over 3 months would make possible to propose specific trials for this condition. Steroid-independence, H3 mutation status and LPS should be considered to predict eligibility.
M. Lobon-Iglesias, G. Giraud, D. Castel, C. Philippe, M. Debily, et al.. Diffuse intrinsic pontine gliomas (DIPG) at recurrence: is there a window to test new therapies in some patients?. Journal of Neuro-Oncology, 2017, 137 (1), pp.111-118. ⟨10.1007/s11060-017-2702-7⟩. ⟨hal-04515971⟩
Adil Mouahid, Cyril Dufour, Elisabeth Badens. Supercritical CO 2 extraction from endemic Corsican plants; comparison of oil composition and extraction yield with hydrodistillation method. Journal of CO2 Utilization, 2017, 20, pp.263 - 273. ⟨10.1016/j.jcou.2017.06.003⟩. ⟨hal-01596432⟩ Plus de détails...
Generally speaking, essential oils (EOs) and components of interest are extracted from plants using hydrodistillation (HD), steam distillation or organic solvent methods The Supercritical CO2 (SC-CO2) extraction technique is a good alternative to the three previously mentioned methods as it is able to be applied at temperatures close to ambient and shows no toxicity to humans or the environment. The aim of this study is to investigate the ability of supercritical CO2 extraction to extract bioactive components from four Corsican endemic plants: Rosmarinus officinalis, Juniperus communis ssp nana, Helichrysum italicum and Pistacia lentiscus. After the extracts were analyzed by gas chromatography, it would appear that SC-CO2 is the most adapted process for the extraction of such components of interest as verbenone, germacrene D, bornyl acetate, ferruginol, transcaryophyllene, elemol,gamma-cadinene, geraniol or beta-eudesmol in higher quantity. The SC-CO2 extraction curves were obtained using two models published by Sovova. These models give access to complementary information and help to estimate the values of some important data such as the possible maximal extraction yield for Helichrysum italicum and the end of the extraction period.
Adil Mouahid, Cyril Dufour, Elisabeth Badens. Supercritical CO 2 extraction from endemic Corsican plants; comparison of oil composition and extraction yield with hydrodistillation method. Journal of CO2 Utilization, 2017, 20, pp.263 - 273. ⟨10.1016/j.jcou.2017.06.003⟩. ⟨hal-01596432⟩
C. Dufour, Christelle Crampon, C. Delbecque, P-P. Garry, Elisabeth Badens. Purification of sclareol by supercritical CO2 fractionation process. Journal of Supercritical Fluids, 2017, 122, pp.35 - 42. ⟨10.1016/j.supflu.2016.12.001⟩. ⟨hal-01596425⟩ Plus de détails...
The implementation and optimization of continuous supercritical CO2 fractionation of a clary sage extract containing 25 wt% of sclareol were performed in order to increase its sclareol content. After preliminary experiments confirming the feasibility of the process, different operating conditions were studied: CO(2-)over-feed mass ratio from 25 up to 115, and pressure between 11 and 13 MPa, with an internal reflux due to a thermal gradient 323-338 K along the column, through an experimental design. Four responses were highlighted: sclareol mass fraction in the raffinate, sclareol mass fraction in the extract, sclareol yield in the raffinate, and the amount of an undesirable compound exhibiting a similar behaviour to sclareol and thus, hard to separate from sclareol by conventional techniques. This work allowed us to highlight the best operating conditions: a pressure of 12.6 MPa and a CO2-over-feed mass ratio of 114.5 leading to a sclareol mass fraction in raffinate and extract of 75.3% and 4.6% respectively, to a sclareol yield of 82.4% in the raffinate, and to an amount of 0.08% of the undesirable compound in the raffinate.
C. Dufour, Christelle Crampon, C. Delbecque, P-P. Garry, Elisabeth Badens. Purification of sclareol by supercritical CO2 fractionation process. Journal of Supercritical Fluids, 2017, 122, pp.35 - 42. ⟨10.1016/j.supflu.2016.12.001⟩. ⟨hal-01596425⟩
